Composite

Part:BBa_K3409008:Design

Designed by: Lucie Pesenti   Group: iGEM20_Ionis_Paris   (2020-10-20)


Expression of receptor binding protein (gp37) on the outer membrane of Escherichia coli with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4480
    Illegal NheI site found at 4503
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1555
    Illegal BglII site found at 2749
    Illegal BglII site found at 4526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 438
    Illegal NgoMIV site found at 2929
    Illegal AgeI site found at 1112
    Illegal AgeI site found at 1224
    Illegal AgeI site found at 1897
    Illegal AgeI site found at 4581
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3132


Design Notes

Synthesis provided by IDT


Source

This is a composite part that includes: Promoter (BBa_J23104), Ribosome binding site (RBS - BBa_B0030), Co-expressed lipoprotein with the outer membrane protein A (Lpp-OmpA - BBa_K1694002), Short linker (Gly-Gly-Ser-Gly) to fuse the proteins (BBa_K243004), TEV cleavage site (BBa_J18918), 10xHis-tag (BBa_K844000), Red fluorescent protein (RFP - BBa_E1010), Gene product (gp) 37 (BBa_K3409000), Double-terminator (BBa_B0015) Promoter medium (BBa_J23107), Ribosome binding site (RBS - BBa_B0032), chaperone protein gp38 (BBa_K3409001), chaperone protein 57A (BBa_K3409002), Terminator (BBa_B1005).

References